|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_076970, 13129044|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 26 kDa |
Observed: 25 kDa
|Application Notes||PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry at 5 µg/mL. For Immunoflorescence start at 20 µg/mL.|
|Target/Specificity||PDCL3; PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.|
|Reconstitution & Storage||PDCL3 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PDCL3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a chaperone for the angiogenic VEGF receptor KDR/VEGFR2, controlling its abundance and inhibiting its ubiquitination and degradation. Modulates the activation of caspases during apoptosis. Is a substrate for Orgyia pseudotsugata multicapsid polyhedrosis virus (OpMNPV) IAP-mediated ubiquitination.|
|Tissue Location||Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine and colon.|
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Provided below are standard protocols that you may find useful for product applications.
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1,2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).
Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5.
Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30.
Lou X, Bao R, Zhou CZ, et al. Structure of the thioredoxin-fold domain of human phosducin-like protein 2. Acta Crystallographica 2009; F65:67-70.
Stirling PC, Srayko M, Takhar KS, et al. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeltal protein function and cell cycle progrssion. Mol. Biol. Cell 2007; 18:2336-45.
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