|Application ||WB, IF, ICC, E|
|Other Accession||NP_919258, 132660|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 88 kDa |
Observed: 82 kDa
|Application Notes||LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL|
|Target/Specificity||LIN54 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human LIN54.|
The immunogen is located within the last 50 amino acids of LIN54.
|Reconstitution & Storage||LIN54 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||LIN54 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context (PubMed:17671431, PubMed:17531812). In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes (PubMed:17671431, PubMed:17531812). In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation (PubMed:17671431, PubMed:17531812). In the complex, acts as a DNA- binding protein that binds the promoter of CDK1 in a sequence- specific manner (PubMed:19725879). Specifically recognizes the consensus motif 5'-TTYRAA-3' in target DNA (PubMed:27465258).|
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Provided below are standard protocols that you may find useful for product applications.
LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).
Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16.
Matsuo T, Kuramoto H, Kumazaki T, et al. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression. Cell Cycle 2012; 11:3227-36.
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