|Application ||WB, IF, ICC, E|
|Other Accession||NP_919258, 169234719|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 88 kDa |
Observed: 82 kDa
|Application Notes||LIN54 antibody can be used for detection of LIN54 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunocytochemistry at 10 µg/ml. For immunofluorescence start at 20 µg/mL|
|Target/Specificity||LIN54; LIN54 antibody is human, mouse, and rat reactive. Multiple isoforms of LIN54 are known to exist.|
|Reconstitution & Storage||LIN54 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||LIN54 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
LIN54 is a component of the LIN, or DREAM, complex, an essential regulator of cell cycle genes. This complex functions as a transcriptional repressor by binding to promoters of E2F target genes that regulate the G1/S transition (1). LIN54 is required for cell cycle progression and can bind to the cdc2 promoter in a sequence-specific manner (2).
Schmit F, Cremer S, and Gaubatz S. LIN54 is an essential core subunit of the DREAM/LINC complex that binds to the cdc2 promotoer in a sequence-specific manner. FEBS J. 2009; 276:5703-16.
Matsuo T, Kuramoto H, Kumazaki T, et al. LIN54 harboring a mutation in CHC domain is localized to the cytoplasm and inhibits cell cycle progression. Cell Cycle 2012; 11:3227-36.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.