|Application ||WB, IF, E|
|Other Accession||NP_001031, 6462|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 25, 26, 31, 32, 38, 44 kDa |
Observed: 35 kDa
|Application Notes||SHBG antibody can be used for detection of SHBG by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||SHBG antibody was raised against a 16 amino acid peptide near the center of human SHBG.|
The immunogen is located within amino acids 220 - 270 of SHBG.
|Reconstitution & Storage||SHBG antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SHBG Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as an androgen transport protein, but may also be involved in receptor mediated processes. Each dimer binds one molecule of steroid. Specific for 5-alpha-dihydrotestosterone, testosterone, and 17-beta-estradiol. Regulates the plasma metabolic clearance rate of steroid hormones by controlling their plasma concentration.|
|Cellular Location||Secreted. Note=In testis, it is synthesized by the Sertoli cells, secreted into the lumen of the seminiferous tubule and transported to the epididymis|
|Tissue Location||Isoform 1 and isoform 2 are present in liver and testis|
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Provided below are standard protocols that you may find useful for product applications.
SHBG is a steroid binding protein that was first described as a plasma protein secreted by the liver and is thought to participate in the regulation of steroid responses. SHBG transports androgens and estrogens in the blood, binding each steroid molecule as a dimer formed from identical or nearly identical monomers (1). Low plasma SHBG levels are associated with obesity, abdominal adiposity, metabolic syndrome, and predict the development of type 2 diabetes (2,3). Polymorphisms in this gene have been associated with polycystic ovary syndrome and type 2 diabetes mellitus (4).
Siiteri PK, Murai JT, Hammond GL, et al. The serum transport of steroid hormones. Recent Prog. Horm. Res. 1982; 38:457-510.
Li C, Ford ES, Li B, et al. Association of testosterone and sex hormone-binding globulin with metabolic syndrome and insulin resistance in men. Diabetes Care 2010; 33:1618-24.
Ding EL, Song Y, Manson JE, et al. Sex hormone-binding globulin and risk of type 2 diabetes in women and men. N. Engl. J. Med. 2009; 361:1152-63.
Hacihanefioglu B, Aybey B, Hakan Ozon Y, et al. Association of anthropometric, androgenic and insulin-related features with polymorphisms in exon 8 of SHBG gene in women with polycystic ovary syndrome. Gynecol. Endocrinol. 2013; 29:361-4.
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