|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_003877, 21493037|
|Calculated MW||Predicted: 94 kDa |
Observed: 100 kDa
|Application Notes||AKAP4 antibody can be used for detection of AKAP4 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||AKAP4; AKAP4 antibody is human specific. At least three isoforms are known to exist; this antibody will detect the two longest isoforms.|
|Reconstitution & Storage||AKAP4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||AKAP4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Major structural component of sperm fibrous sheath. Plays a role in sperm motility.|
|Cellular Location||Cell projection, cilium, flagellum. Note=Localizes to the principle piece of the sperm flagellum|
|Tissue Location||Testis specific; only expressed in round spermatids.|
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Provided below are standard protocols that you may find useful for product applications.
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell (1). AKAP4, also known as hAKAP82, is a major sperm fibrous sheath protein that localizes to the entire length of the flagellum of human sperm (2). It has been suggested that AKAP4 plays multiple roles in sperm motility and regulation of signal transduction pathways (2). AKAP4 has also been postulated to be a potential biomarker for breast cancer (3).
Carnegie GK, Means CK, and Scott JD. A-kinase anchoring proteins: from protein complexes to physiology and disease. IUBMB 2009; 61:394-406.
Turner RM, Johnson LR, Haig-Ladewig L, et al. An X-linked gene encodes a major human sperm fibrous sheath protein, hAKAP82. Genomic organization, protein kinase A-RII binding, and distribution of the precursor in the sperm tail. J. Biol. Chem. 1998; 273:32135-41.
Saini S, Jagadish N, Gupta A, et al. A novel cancer testis antigen, A-kinase anchor protein 4 (AKAP4) is a potential biomarker for breast cancer. PLoS One 2013; 8:e57095.
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