|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_060523, 24431977|
|Calculated MW||Predicted: 43 kDa |
Observed: 47 kDa
|Application Notes||XKR8 antibody can be used for detection of XKR8 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||XKR8; XKR8 antibody is human reactive. At least two isoforms are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||XKR8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||XKR8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Promotes phosphatidylserine exposure on apoptotic cell surface, possibly by mediating phospholipid scrambling. Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment. Has no effect on calcium-induced exposure of phosphatidylserine. Activated upon caspase cleavage, suggesting that it does not act prior the onset of apoptosis.|
|Cellular Location||Cell membrane; Multi-pass membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
The XK Kell blood group complex subunit-related family member 8 (XKR8) is a member of a family of proteins that promote the phagocytosis of dying cells by altering the phospholipid distribution of the plasma membrane (1). XKR8 is thought to be a “scramblase”, an enzyme that eliminates the asymmetry of phospholipid distribution in the plasma membrane. In apoptotic cells, XKR8 promotes the cell-surface exposure of phosphatidylserine (PtdSer) which serves as a signal for phagocytosis (1,2).
Suzuki J, Denning DP, Imanishi E, et al. Xk-related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells. Science 2013; 341:403-6.
Wang X, Wu YC, Fadok VA, et al. Cell corpse engulfment mediated by C. elegans phosphatidylserine receptor through CED-5 and CED-12. Science 2003; 302:1563-6.
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