|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_004139, 148922911|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 17 kDa |
Observed: 16 kDa
|Application Notes||NINJ1 antibody can be used for detection of NINJ1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||NINJ1; NINJ1 antibody is human, mouse and rat reactive.|
|Reconstitution & Storage||NINJ1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||NINJ1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Homophilic cell adhesion molecule that promotes axonal growth. May play a role in nerve regeneration and in the formation and function of other tissues. Cell adhesion requires divalent cations.|
|Cellular Location||Membrane; Multi-pass membrane protein.|
|Tissue Location||Widely expressed in both adult and embryonic tissues, primarily those of epithelial origin|
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Provided below are standard protocols that you may find useful for product applications.
Ninjurin-1 (NINJ1) is a homophilic adhesion molecule that is strongly induced in dorsal root ganglion (DRG) and Schwann cells following nerve injury and promotes neurite outgrowth of DRG neurons (1,2), suggesting that NINJ1 may play a role in nerve regeneration. NINJ1 is transcriptionally regulated by p53 and is induced by DNA damage in a p53-dependent manner. A deficiency of NINJ1 increases p53 expression, suppresses cell proliferation, but enhances apoptosis and premature senescence in a p53-dependent manner as well as radiation-induced cell mortality (3).
Araki T and Milbrandt J. Ninjurin, a novel adhesion molecule, is induced by nerve injury and promotes axonal growth. Neuron 1996; 17:353-61.
Araki T, Zimonjic DB, Popescu NC, et al. Mechanism of homophilic binding mediated by ninjurin, a novel widely expressed adhesion molecule. J. Biol. Chem. 1997; 212:21373-80.
Cho SJ, Rossi A, Jung YS, et al. Ninjurin1, a target of p53, regulates p53 expression and p53-dependent cell survival, sensecence, and radiation-induced mortality. Proc. Natl. Acad. Sci. USA 2013; 110:9362-7.
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