|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_008860, 67782311|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 113, 137 kDa |
Observed: 135 kDa
|Application Notes||SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SKIV2L; SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.|
|Reconstitution & Storage||SKI2W antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SKI2W Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||DDX13, SKI2W, SKIV2, W|
|Function||Helicase; has ATPase activity. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C).|
|Cellular Location||Nucleus. Cytoplasm|
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Provided below are standard protocols that you may find useful for product applications.
SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1,2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3,4).
Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6.
Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77.
Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47.
Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.
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