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P2RX7 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, ICC, E |
|---|---|
| Primary Accession | Q99572 |
| Other Accession | NP_002553, 300068987 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 34, 47, 65 kDa Observed: 54 kDa |
| Application Notes | P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
| Gene ID | 5027 |
|---|---|
| Target/Specificity | P2RX7; P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist. |
| Reconstitution & Storage | P2RX7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | P2RX7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | P2RX7 |
|---|---|
| Function | Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206). |
| Cellular Location | Cell membrane; Multi-pass membrane protein |
| Tissue Location | Widely expressed with highest levels in brain and immune tissues. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).
References
Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8.
North RA. Molecular physiology of P2X receptors. Physiol. Rev. 2002; 82:1013-67.
Labasi JM, Petrushova N, Donovan C, et al. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J. Immunol. 168:6436-45.
Basso AM, Bratcher NA, Harris RR, et al. Behavioral profile of P2X7 receptor knockout mice in animal models of depression and anxiety; relevance for neuropsychiatric disorders. Behav. Brain Res. 2009; 198:83-90.
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