|Application ||WB, IF, ICC, E|
|Other Accession||NP_002553, 300068987|
|Calculated MW||Predicted: 34, 47, 65 kDa |
Observed: 54 kDa
|Application Notes||P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunocytochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||P2RX7; P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.|
|Reconstitution & Storage||P2RX7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||P2RX7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells. In the absence of its natural ligand, ATP, functions as a scavenger receptor in the recognition and engulfment of apoptotic cells (PubMed:21821797, PubMed:23303206).|
|Cellular Location||Cell membrane; Multi- pass membrane protein|
|Tissue Location||Widely expressed with highest levels in brain and immune tissues.|
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Provided below are standard protocols that you may find useful for product applications.
The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).
Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8.
North RA. Molecular physiology of P2X receptors. Physiol. Rev. 2002; 82:1013-67.
Labasi JM, Petrushova N, Donovan C, et al. Absence of the P2X7 receptor alters leukocyte function and attenuates an inflammatory response. J. Immunol. 168:6436-45.
Basso AM, Bratcher NA, Harris RR, et al. Behavioral profile of P2X7 receptor knockout mice in animal models of depression and anxiety; relevance for neuropsychiatric disorders. Behav. Brain Res. 2009; 198:83-90.
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