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>   home   >   Products   >   Primary Antibodies   >   Stem Cells   >   HES5 Antibody   

HES5 Antibody

     
  • WB - HES5 Antibody ASC11878
    Western blot analysis of PTEN in human brain tissue lysate with PTEN antibody at (A) 1 and (B) 2 µg/mL..
  • WB - HES5 Antibody ASC11878
    Western blot analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 µg/mL.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession Q5TA89
Other Accession NP_001010926, 388585
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 18 kDa

Observed: 17 kDa
Application Notes HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Additional Information
Gene ID 388585
Target/Specificity HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5.

The immunogen is located within the first 50 amino acids of HES5.
Reconstitution & Storage HES5 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsHES5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HES5
Synonyms BHLHB38
Function Transcriptional repressor of genes that require a bHLH protein for their transcription. Plays an important role as neurogenesis negative regulator (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00380, ECO:0000255|PROSITE-ProRule:PRU00981}
Tissue Location Expressed in fetal heart and brain tumors.
Research Areas
Citations (0)

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Background

HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).

References

Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.

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$ 320.00
Cat# ASC11878
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