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ATG4D Antibody

  • WB - ATG4D Antibody ASC11885
    Western blot analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 µg/ml.
  • IHC - ATG4D Antibody ASC11885
    Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 µg/ml.
  • IF - ATG4D Antibody ASC11885
    Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 µg/ml.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q86TL0
Other Accession NP_116274, 27903825
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype IgG
Calculated MW Predicted: 52 kDa

Observed: 49 kDa
Application Notes ATG4D antibody can be used for detection of ATG4D by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.
Additional Information
Gene ID 84971
Target/Specificity ATG4D; ATG4D antibody is human, mouse and rat reactive. At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins.
Reconstitution & Storage ATG4D antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsATG4D Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATG4D
Synonyms APG4D, AUTL4
Function Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Cellular Location Cysteine protease ATG4D: Cytoplasm.
Tissue Location Mainly expressed in skeletal muscle and, to a lower extent, in testis.
Research Areas
Citations (0)

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Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4D, also known as AUTL4, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).


Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
Marino G, Uria JA, Puente XS, et al. Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy. J. Biol. Chem. 2003; 278:3671-8.
Li M, Hou Y, Wang J, et al. Kinetic comparisons of mammalian Atg4 homologues indicate selective preferences towards diverse Atg8 substrates. J. Biol. Chem. 2011; 286:7327-38.

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$ 310.00
Cat# ASC11885
(40 western blots)
Availability: 5-7days
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