|Application ||WB, IHC-P, IF, E|
|Other Accession||NP_001274524, 567316146|
|Calculated MW||Predicted: 17, 52, 62, 68 kDa |
Observed: 52 kDa
|Application Notes||SLC1A7 antibody can be used for detection of SLC1A7 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||SLC1A7; SLC1A7 antibody is human and mouse reactive. Multiple isoforms of SLC1A7 are known to exist.|
|Reconstitution & Storage||SLC1A7 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SLC1A7 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transports L-glutamate; the L-glutamate uptake is sodium- and voltage-dependent and chloride-independent. Its associated chloride conductance may participate in visual processing.|
|Cellular Location||Membrane; Multi-pass membrane protein.|
|Tissue Location||Expressed primarily in retina. Detectable in liver, heart, muscle and brain|
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Provided below are standard protocols that you may find useful for product applications.
The solute carrier family 1 member 7 (SLC1A7) protein was initially identified as a glutamate transporter coupled to a chloride conductance that is expressed primarily in the retina (1). More recent evidence indicates that is it widely expressed in peripheral tissues, also (2). Experiments have shown that SLC1A7 functions as a low-affinity/low-capacity glutamate transport, with an anion channel optimized for anion conductance in the negative voltage range (3). In the retina, SLC1A7 co-localizes with the serum and glucocorticoid dependent kinase SGK1, and its cell surface expression and activity is regulated SGK1 and its isoform SGK3 (4).
Arriza JL, Eliasof S, Kavanaugh MP, et al. Excitatory amino acid transporter 5, a retinal glutamate transporter coupled to a chloride conductance. Proc. Natl. Acad. Sci. USA 1997; 94:4155-60.
Lee A, Anderson AR, Stevens M, et al. Excitatory amino acid transporter 5 is widely expressed in peripheral tissues. Eur. J. Histochem. 2013; 57:e11.
Schneider N, Cordeiro S, Machtens JP, et al. Functional properties of the retinal glutamate transporters GLT-1c and EAAT5. J. Biol. Chem. 2014; 289:1815-24.
Boehmer C, Rajamanickam J, Schniepp R, et al. Regulation of the excitatory amino acid transporter EAAT5 by the serum and glucocorticoid dependent kinases SGK1 and SGK3. Biochem. Biophys. Res. Commun. 2005; 329:738-42.
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