|Application ||WB, IF, E|
|Other Accession||NP_060496, 30089916|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 106 kDa; Observed: 105 kDa|
|Application Notes||PACS1 antibody can be used for detection of PACS1 by Western blot at 1 - 2 µg/ml. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||PACS1; PACS1 antibody is human, mouse and rat reactive. Multiple isoforms of PACS1 are known to exist. PACS1 antibody is predicted to not cross-react with PACS2.|
|Reconstitution & Storage||PACS1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||PACS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Coat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin- coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN.|
|Cellular Location||Golgi apparatus, trans-Golgi network. Note=Localizes in the perinuclear region, probably the TGN.|
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Provided below are standard protocols that you may find useful for product applications.
The phosphofurin acidic cluster sorting protein 1 (PACS1), belongs to the PACS family of cytosolic connector proteins that localize different membrane proteins to the trans-Golgi network (TGN) (1,2). Mutations in PACS1 cause defects in cranial-neural crest migration during early development, leading to a recognizable intellectual disability syndrome (3). PACS1, along with the related protein PACS2, also plays a role in HIV-1 Nef-mediated downregulation of cell surface MHC-I molecules to the TGN, thereby enabling HIV-1 to escape immune surveillance (4).
Wan L, Molloy SS, Thomas L, et al. PACS-1 defines a novel gene family of cytosolic sorting proteins required for trans-Golgi network localization. Cell 1998; 94:205-16.
Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16.
Schuurs-Hoeijmakers JH, Oh EC, Vissers LE, et al. Recurrent de novo mutations in PACS1 cause defective cranial-neural-crest migration and define a recognizable intellectual-disability syndrome. Am. J. Hum. Genet. 2012; 91:1122-7.
Dikeakos JD, Thomas L, Kwon G, et al. An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS2 on endosomes to down-regulate MHC-I. Mol. Biol. Cell 2012; 23:2184-97.
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