|Application ||WB, IF, ICC, E|
|Other Accession||NP_006437, 10599|
|Calculated MW||Predicted: 76 kDa |
Observed: 75 kDa
|Application Notes||SLCO1B1 antibody can be used for detection of SLCO1B1 by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.|
|Target/Specificity||SLCO1B1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human SLCO1B1.|
The immunogen is located within the last 50 amino acids of SLCO1B1.
|Reconstitution & Storage||SLCO1B1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.|
|Precautions||SLCO1B1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||LST1, OATP1B1, OATP2, OATPC, SLC21A6|
|Function||Mediates the Na(+)-independent uptake of organic anions such as pravastatin, taurocholate, methotrexate, dehydroepiandrosterone sulfate, 17-beta-glucuronosyl estradiol, estrone sulfate, prostaglandin E2, thromboxane B2, leukotriene C3, leukotriene E4, thyroxine and triiodothyronine. Involved in the clearance of bile acids and organic anions from the liver.|
|Cellular Location||Basolateral cell membrane; Multi-pass membrane protein. Note=Detected in basolateral membranes of hepatocytes|
|Tissue Location||Highly expressed in liver, at the basolateral membranes of centrilobular hepatocytes. Not detected in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocyte.|
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Provided below are standard protocols that you may find useful for product applications.
SLCO1B1 is a transmembrane receptor that mediates the sodium-independent uptake of numerous endogenous compounds including bilirubin, 17-beta-glucuronosyl estradiol and may play an important role in the clearance of bile acids and organic anions from the liver (1,2). It contains one Kazal-like domain and belongs to the organo-anion transporter family (2,3). SLCO1B1 is highly expressed in liver and is localized to the basolateral hepatocyte membrane. It is responsible for the hepatic uptake of the liver-specific hydroxymethylglutaryl-CoA reductase inhibitor in mouse, rat and human (3,4).
Abe T, Kakyo M, Tokui T, et al. Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1. J. Biol. Chem. 1999; 274:17159-63.
Konig J, Cui Y, Nies AT, et al. A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane. Am. J. Physiol. Gastrointest. Liver Physiol. 2000; 278:G156-64.
Michalski C, Cui Y, Nies AT, et al. A naturally occurring mutation in the SLC21A6 gene causing impaired membrane localization of the hepatocyte uptake transporter. J. Biol. Chem. 2002; 277:43058-63.
Yao J, Hong W, Huang J, et al. N-Glycosylation dictates proper processing of organic anion transporting polypeptide 1B1. PLoS One 2012; 7:e52563.
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