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RNF121 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, ICC, E |
|---|---|
| Primary Accession | Q9H920 |
| Other Accession | NP_060790, 21361732 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 24, 27, 36 kDa Observed: 28 kDa |
| Application Notes | RNF121 antibody can be used for detection of RNF121 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunocytochemistry at 10 µg/ml. |
| Gene ID | 552978 |
|---|---|
| Target/Specificity | RNF121; RNF121 antibody is human, mouse and rat reactive. Multiple isoforms of RNF121 are known to exist. |
| Reconstitution & Storage | RNF121 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | RNF121 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RNF121 |
|---|---|
| Function | E3 ubiquitin ligase which accepts ubiquitin and transfers it to substrates thereby promoting their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway which is a pathway involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (By similarity). May regulate the unfolded protein response to reduce endoplasmic reticulum stress (By similarity). |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q09251}; Multi-pass membrane protein |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
RNF121 is an evolutionarily conserved E3 ligase ring finger protein that is expressed in the endoplasmic reticulum (1). It acts downstream of the unfolded protein response regulator protein kinase-like endoplasmic reticulum kinase (PERK). Overexpression of RNF121 during the early stages of gonad development led to aberrations in germline development and gonad migration, suggesting that it plays a role in endoplasmic reticulum-associated degradation machinery (1).
References
Darom A, Bening-Abu-Shach U, and Broday L. RNF-121 is an endoplasmic reticulum-membrane E3 ubiquitin ligase involved in the regulation of B-integrin. Mol. Biol. Cell 2010; 21:1788-98.
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