Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
BCLAF1 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, E |
|---|---|
| Primary Accession | Q9NYF8 |
| Other Accession | NP_055554, 7661958 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 101 kDa; Observed: 105 kDa |
| Application Notes | BCLAF1 antibody can be used for detection of BCLAF1 by Western blot at 0.5 - 1 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. |
| Gene ID | 9774 |
|---|---|
| Target/Specificity | BCLAF1; BCLAF1 antibody is human, mouse and rat reactive. At least four isoforms of BCLAF1 are known to exist; this antibody will detect all but isoform 2. |
| Reconstitution & Storage | BCLAF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
| Precautions | BCLAF1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BCLAF1 |
|---|---|
| Synonyms | BTF, KIAA0164 |
| Function | Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. |
| Cellular Location | Cytoplasm. Nucleus. Nucleus speckle. Nucleus, nucleoplasm |
| Tissue Location | Ubiquitous. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The BCL2-associated transcription factor 1 (BCLAF1) is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (1). BCLAF1 localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. (2). More recent studies have suggested BCLAF1 plays roles in processes not associated with actions of the BCL2 family members, including lung development, T cell activation, and post-transcriptional processes that affect mRNA metabolism (3).
References
Kasof GM, Goyal L, and White E. Btf, a novel death-promoting transcriptional repressor that interacts with Bcl-2-related proteins. Mol. Cell Biol. 1999; 19:4390-404.
Haraguchi T, holaska JM, Yamana M, et al. Emerin binding to Btf, a death-promoting transcriptional repressor, is disrupted by a missense mutation that causes Emery-Dreifuss muscular dystrophy. Eur. J. Biochem. 2004; 271:1035-45.
Sarras H, Azami SA, and McPherson JP. In search of a function for BCLAF1. The Scientific World Journal 2010; 10:1450-61.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
