|Application ||WB, IHC-P, IF, E|
|Other Accession||Q96D31, 97180269|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||Predicted: 33 kDa |
Observed: 50 kDa
|Application Notes||ORAI1 antibody can be used for detection of ORAI1 by Western blot at 1 - 2 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.|
|Target/Specificity||ORAI1; ORAI1 antibody is predicted to have no cross-reactivity to ORAI2 or ORAI3.|
|Reconstitution & Storage||ORAI1 monoclonal antibody can be stored at -20℃, stable for one year.|
|Precautions||ORAI1 Antibody [6D11A11] is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor, STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the immune response to pathogens by activating the transcription factor NFAT.|
|Cellular Location||Cell membrane; Multi- pass membrane protein Note=Isoform beta is more mobile in the plasma membrane|
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Provided below are standard protocols that you may find useful for product applications.
ORAI1 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI1 is a recently identified four-transmembrane spanning protein that is an essential component of CRAC. A missense mutation in this protein in humans is the cause of one form of hereditary severe combined immune deficiency (SCID) which results in ablated T-cell Ca++ entry. It has been suggested that ORAI1 functions as a highly selective Ca++ plasma membrane channel that is gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor.
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521.
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85.
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8.
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