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Lin28 Antibody [1G9H9]

  • WB - Lin28 Antibody [1G9H9]  ASC12018
    Western blot analysis of Lin28 in Raji cell lysate with Lin28 antibody at 0.5 µg/mL in (A) the absence and (B) the presence of blocking peptide.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H9Z2
Other Accession NP_078950, 13375938
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Clone Names 1G9H9
Calculated MW Predicted: 23 kDa
Application Notes Lin28 antibody can be used for detection of Lin28 by Western blot at 0.5 - 1 µg/mL.
Additional Information
Gene ID 79727
Target/Specificity LIN28A; At least two isoforms of Lin28 are known to exist; this antibody will detect both. Lin28 antibody will not cross-react with Lin28 Homolog B.
Reconstitution & Storage Lin28 Monoclonal antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
PrecautionsLin28 Antibody [1G9H9] is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name LIN28A
Synonyms CSDD1, LIN28, ZCCHC1
Function Acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. Its association with the translational machinery and target mRNAs results in an increased number of initiation events per molecule of mRNA and, indirectly, in stabilizing the mRNAs. Binds IGF2 mRNA, MYOD1 mRNA, ARBP/36B4 ribosomal protein mRNA and its own mRNA. Essential for skeletal muscle differentiation program through the translational up-regulation of IGF2 expression (By similarity). Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let- 7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre- let-7 in embryonic stem (ES) cells contributes to the maintenance of ES cells. In contrast, LIN28A down-regulation in neural stem cells by miR-125, allows the processing of pre-let-7. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation.
Cellular Location Cytoplasm. Nucleus, nucleolus. Note=Nucleolar localization observed in 10-15% of the nuclei in differentiated myotubes (By similarity). Shuttles between the cytoplasm and the nucleus. Localizes to cytoplasmic processing bodies and stress granules.
Tissue Location Expressed in embryonic stem cells (ES cells), placenta and testis.
Research Areas
Citations (0)

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Lin28 Monoclonal Antibody: Lin28 is a transcription factor that was first identified through its key role in the pathway of developmental timing in C. elegans. The role of Lin28 in development suggested that it might be useful in the creation of stem cells that might be beneficial in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing Lin28 in addition to the transcription factors POU5F1, Sox2, and NANOG in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing Lin28, Sox2, POU5F1 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.


Ambros V. A hierarchy of regulatory genes controls a larva-to-adult developmental switch in C. elegans. Cell 1989; 57:49-57.
Carpenter MK, Rosler E, and Rao MS. Characterization and differentiation of human embryonic stem cells. Cloning Stem Cells 2003; 5:79-88.
Tyu J, Vodyanik MA, Smuga-Otto K, et al. Induced pluripotent stem cell lines derived from human somatic cells. Science 2007; 318:1917-20
Okita K, Nakagawa M, Hyenjong H, et al. Generation of mouse induced pluripotent stem cells without viral vectors. Science 2008; 322:949-53.

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$ 310.00
Cat# ASC12018
(40 western blots)
Availability: 5-7days
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