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Acetylated Lysine Antibody
Acetylated Lysine Antibody, Clone 7F8
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, ICC, IP, E |
|---|---|
| Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Description | Mouse Anti- Acetylated Lysine Monoclonal IgG1 |
| Target/Specificity | Detects proteins containing acetylated lysine residues in ELISA and western blots. Does not detect non-acetylated lysine residues. |
| Other Names | Acetyl Lysine Antibody, Acetylated lysine Antibody, Lysine Antibody |
| Clone Names | 7F8 |
| Immunogen | Acetylated KLH |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg of SMC-153 was sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein, on western blot by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Nucleus |
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Provided below are standard protocols that you may find useful for product applications.
Background
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common post-transcriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε development (5).
References
1. Yang XJ. (2005). Oncogene. 24:1653-1662.
2. Hassig, C.A. and Schreiber, S.L. (1997). Curr. Opin. Chem. Biol. 1(3): 300-308.
3. Yang XJ. (2004). Bioessays 26:1076-1087.
4. Hughes, R.E. (2002). Curr. Biol. 12: R141-R143.
5. Vigushin, D.M. and Coombes, R.C. (2004). Curr. Cancer Drug Targets 4: 205-218.
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