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7-Ketocholesterol Antibody

7-Ketocholesterol Antibody, Clone 3F7

     
  • ICC/IF - 7-Ketocholesterol Antibody ASM10341
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (ASM10341). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (ASM10341) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 7-Ketocholesterol Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
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  • WB - 7-Ketocholesterol Antibody ASM10341
    Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol-BSA using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (ASM10341). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: BSA (2.0 µg). Lane 4: 7-ketocholesterol-BSA (0.5 µg). Lane 5: 7-ketocholesterol-BSA (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (ASM10341) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, ICC, E
Host Mouse
Isotype IgG3
Clonality Monoclonal
Description Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG3
Target/Specificity Specific for 7-ketocholesterol modified proteins.
Other Names 7-Ketocholesterol [7-KC] Antibody, 7-KC Antibody, 7-ketocholesterol (7-KC) Antibody, 7KC Antibody, 7KC-Modified Keyhole-Lympet Hemocyanine Antibody, 7KC-Modified KLH Antibody
Trademark MOLECULAR SIGNATURE®
Clone Names 3F7
Immunogen Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
Purification Protein G Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.9% Sodium Azide
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SMC-513 was sufficient for detection of 7-Ketocholesterol in 2 µg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
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Cat# ASM10341
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