|Description||Anti-HUMAN IgM (mu chain) (GOAT) Antibody|
|Application ||WB, E, IC|
|Application Note||ELISA 1:10,000-1:50,000|
Western Blot 1:1,000-1:5,000
|Physical State||Liquid (sterile filtered)|
|Target Isotype||IgM µ chain|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Anti-Human IgM mu heavy chain was produced by repeated immunization with Human IgM mu heavy chain in goat.|
|Preservative||0.01% (w/v) Sodium Azide|
|Shipping Condition||Wet Ice|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.|
|Storage Condition||Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Anti-Human IgM mu heavy chain antibody generated in goat detects specifically Human IgM mu heavy chain. This secondary antibody anti-Human is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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