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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-MOUSE IgG (H&L) Secondary Antibody   

Anti-MOUSE IgG (H&L) Secondary Antibody

Goat Polyclonal, Unconjugated

  • WB - Anti-MOUSE IgG (H&L)  Secondary Antibody ASR1149
    Western Blot of Goat anti-Mouse IgG (H&L) Antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 100 ng per lane. Primary Antibody: Mouse IgG (H&L) Antibody 1:1000 overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Obsevered Size: 28 and 55 kDa/28 and 55 kDa for Mouse IgG. Other band(s): none.
  • WB - Anti-MOUSE IgG (H&L)  Secondary Antibody ASR1149
    Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule. Lane 2: Mouse IgG F(c) Fragment. Lane 3: Mouse IgG F(ab) Fragment. Lane 4: Mouse IgM Kappa. Lane 5: Mouse Serum. Load: 50ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG 1:40,000 for 30 min at RT . Predicted/Obsevered Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.
Product Information
Description Anti-MOUSE IgG (H&L) (GOAT) Antibody
Host Goat
Conjugate Unconjugated
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:150,000
IF Microscopy Yes
FlowCytometry Yes
Western Blot 1:3,000-1:15,000
Immunochemistry 1:1,000-1:5,000ImmunoPrecipitation:Yes
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Secondary Antibody Anti-Mouse IgG (H&L) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum.
Storage Condition Store Anti-Mouse Secondary Antibody at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Anti-Mouse IgG affinity purified secondary antibody is generated in goat detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

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$ 107.00
Cat# ASR1149
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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