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Anti-Rat IgG (H&L) (Biotin Conjugated) Secondary Antibody

Goat Polyclonal, Biotin

  • WB - Anti-Rat IgG (H&L)  (Biotin Conjugated) Secondary Antibody ASR1652
    Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. Load: 100 ng per lane. Primary antibody: Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT. Secondary antibody: HRP Conjugated Streptavidin at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT.
  • WB - Anti-Rat IgG (H&L)  (Biotin Conjugated) Secondary Antibody ASR1652
    Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product Information
Description Anti-RAT IgG (H&L) (GOAT) Antibody Biotin Conjugated
Host Goat
Conjugate Biotin
Target Species Rat
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:10,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Rat IgG whole molecule
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity Anti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum.
Storage Condition Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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RAT IgG Biotin Conjugated antibody detects rat immunoglobulin G. Immunoglobuoin G is a molecule of about 150 kDa composed of four peptide chains. Each IgG contains two identical ? heavy chains of about 51 kDa and two identical light chains of about 26 kDa, thus a tetrameric quaternary structure. The two heavy chains are linked to each other and to a light chain each by disulfide bonds. The resulting tetramer has two identical halves, which together form the Y-like shape. Each end of the fork contains an identical antigen binding site. The Fc regions of IgGs bear a highly conserved N-glycosylation site.

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$ 128.00
Cat# ASR1652
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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