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Anti-MOUSE IgG (H&L) (Peroxidase Conjugated) Secondary Antibody
Rabbit Polyclonal, Peroxidase (Horseradish)
- SPECIFICATION
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| Description | Anti-MOUSE IgG (H&L) (RABBIT) Antibody Peroxidase Conjugated |
|---|---|
| Host | Rabbit |
| Conjugate | Peroxidase (Horseradish) |
| Target Species | Mouse |
| Clonality | Polyclonal |
Application 
| WB, E, IC |
| Application Note | ELISA 1:50,000-1:300,000 Western Blot 1:1,000-1:5,000 Immunochemistry 1:500-1:2,500 |
| Physical State | Lyophilized |
| Host Isotype | IgG |
| Target Isotype | IgG (H&L) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Mouse IgG whole molecule |
| Reconstitution Volume | 1.0 mL |
| Reconstitution Buffer | Restore with deionized water (or equivalent) |
| Stabilizer | 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Shipping Condition | Ambient |
|---|---|
| Purity | HRP Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. |
| Storage Condition | Store mouse second antibody conjugate at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mouse secondary antibody conjugated to horseradish peroxidase is available in a variety of formats. Anti-IgG Secondary Antibody conjugate is Suitable for western blot, ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
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