|Description||F(ab')2 Anti-MOUSE IgG F(c) (GOAT) Antibody Peroxidase Conjugated Min X Bv Hs & Hu Serum Proteins|
|Application ||WB, E, IC|
|Application Note||ELISA 1:100,000|
Western Blot 1:500-1:2,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG F(c)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Mouse IgG F(c) fragment|
|Reconstitution Volume||500 µL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!|
|Purity||F(ab')2 Anti-Mouse IgG F(c) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab’)2 or Bovine, Horse, and Human Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
F(ab')2 Anti-Mouse IgG F(c) Antibody generated in goat specifically detects Mouse IgG F(ab')2. F(ab')2 Anti-Mouse IgG F(c)Antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. F(ab')2 Anti-Mouse IgG F(c) Antibody is ideal for investigators in Cancer, Immunology, and Microbiology research.
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