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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Rat IgG F(c)    

Rat IgG F(c)

  •  - Rat IgG F(c)  ASR2574
    SDS-PAGE of Rat IgG F(c) Fragment . Lane M: 3 µL Opal Prestained Marker . Lane 1: Reduced Rat IgG Whole Molecule . Lane 2: Reduced Rat IgG F(c) Fragment . Lane 3: Reduced Rat IgG F(ab) Fragment . Lane 4: Reduced Rat IgM Whole Molecule . Load: 1 µg of IgG, F(c) and F(ab); 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.
  •  - Rat IgG F(c)  ASR2574
    SDS-Page of Rat IgG F(c). Lane 1: Rat Fc – Non-reduced. Lane 2: Rat Fc - Reduced. Load: 1.0 µg per lane. Predicted/Observed size: 25 kDa, 25 kDa for Reduced Fc. Other band(s): None.
Product Information
Description RAT IgG F(c) fragment
Conjugate Unconjugated
Physical State Liquid (sterile filtered)
Host Isotype IgG F(c)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Species of Origin Rat
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c).  No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain.
Storage Condition Store vial at 4° C prior to restoration.   Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Rat IgG Fc fragment is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(c) fragment binds with very high affinity to the Fc receptor proteins on phagocytic leukocytes. When digested from the whole antibody molecule, the F(c) fragment no longer posses the epitope recognition site.

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$ 192.00
Cat# ASR2574
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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