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>   home   >   Products   >   Secondary Antibodies   >   R-Phycoerythrin (RPE)   >   Anti-Mouse IgG1 (Peroxidase Conjugated) Pre-adsorbed Secondary Antibody   

Anti-Mouse IgG1 (Peroxidase Conjugated) Pre-adsorbed Secondary Antibody

Goat Polyclonal, Peroxidase (Horseradish)

     
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Product Information
Description Anti-MOUSE IgG1 (Gamma 1 chain) (GOAT) Antibody Peroxidase Conjugated (Min Cross Bv, Hu, and Rb Serum Proteins)
Host Goat
Conjugate Peroxidase (Horseradish)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:20,000-1:100,000
Western Blot 1:2,000-1:20,000
Immunochemistry 1:1,000-1:5,000
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG1
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG1 heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Additional Information
Shipping Condition Ambient
Purity Anti-Mouse IgG1 HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG1 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse Serum and Mouse IgG. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
Storage Condition Store vial antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
Citations (0)
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Background

HRP Conjugated Secondary Antibodies are ideal for western blot and are available in a variety of formats and conjugate types. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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$ 210.00
Cat# ASR2717
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