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>   home   >   Products   >   Secondary Antibodies   >   Texas Red®   >   Anti-Mouse IgG1 (Fluorescein Conjugated) Secondary Antibody   

Anti-Mouse IgG1 (Fluorescein Conjugated) Secondary Antibody

Rabbit Polyclonal, Fluorescein (FITC)

  • WB - Anti-Mouse IgG1  (Fluorescein Conjugated) Secondary Antibody ASR2749
    Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG1 (Gamma 1 chain) secondary antibody. Lane 1: Mouse IgG1. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG1 (Gamma 1 chain). Other band(s): None.
Product Information
Description Anti-MOUSE IgG1 (Gamma 1 chain) (RABBIT) Antibody Fluorescein Conjugated
Host Rabbit
Conjugate Fluorescein (FITC)
FP Value 3.9 moles Fluorescein (FITC) per mole of IgG
Target Species Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note FLISA 1:10,000-1:50,000
IF Microscopy 1:500-1:2,500
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG1
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Mouse IgG1 heavy chain
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Storage Condition Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Mouse IgG1 secondary antibody conjugated to FITC is available in a variety of formats. Anti Mouse IgG1 secondary antibody conjugate is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This IgG1 antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

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$ 199.00
Cat# ASR2749
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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