F(ab')2 Anti-Human IgG F(c) (Biotin Conjugated) Pre-Adsorbed Secondary Antibody
Goat Polyclonal, Biotin
|Description||F(ab')2 Anti-HUMAN IgG F(c) (GOAT) Antibody Biotin Conjugated Min X Bv Hs Ms & Rt Serum Proteins|
|Application ||WB, E, IC|
|Application Note||ELISA 1:20,000-1:100,000|
Western Blot 1:2,000-1:10,000
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG F(c)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Immunogen||Human IgG F(c) fragment|
|Reconstitution Volume||1.0 mL|
|Reconstitution Buffer||Restore with deionized water (or equivalent)|
|Stabilizer||10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Human IgG, Human IgG F(c) and Human Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Human IgG F(ab’)2 or Bovine, Horse, Mouse and Rat Serum Proteins.|
|Storage Condition||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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