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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Chicken IgM (mu chain) (Alkaline Phosphatase Conjugated) Secondary Antibody   

Anti-Chicken IgM (mu chain) (Alkaline Phosphatase Conjugated) Secondary Antibody

Goat Polyclonal, Alkaline Phosphatase (Calf Intestine)

  • WB - Anti-Chicken IgM (mu chain)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR3082
    Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated . Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule . Lane 2: Chicken IgG F(c) Fragment . Lane 3: Chicken IgG F(ab) Fragment . Lane 4: Chicken IgM Whole Molecule . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Obsevered Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and F(ab), 75 kDa for IgM. Chicken F(c) migrates slightly higher.
Product Information
Description Anti-CHICKEN IgM (mu chain) (GOAT) Antibody Alkaline Phosphatase Conjugated
Host Goat
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Chicken
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:8,000
Western Blot 1:200-1:2,000
Immunohistochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgM µ chain
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Chicken IgM mu heavy chain
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Chicken IgM and Chicken Serum.  No reaction was observed against Chicken IgG.
Storage Condition Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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$ 252.00
Cat# ASR3082
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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