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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Rat IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody   

Anti-Rat IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

Goat Polyclonal, R-Phycoerythrin (RPE)

  • WB - Anti-Rat IgG (H&L)  (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody ASR3108
    Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) . Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule . Lane 2: Rat IgG F(c) Fragment . Lane 3: Rat IgG F(ab) Fragment . Lane 4: Rat IgM Whole Molecule . Lane 5: Rat Serum . All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in MB-070 for 30 min at RT. Predicted/Obsevered Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and F(ab), 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
Product Information
Description Anti-RAT IgG [H&L] (GOAT) Antibody Phycoerythrin conjugated Min X Bv Ch Gt GP Ham Hs Hu Ms Rb and Sh Serum Proteins
Host Goat
Conjugate R-Phycoerythrin (RPE)
Target Species Rat
Reactivity Rat
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
Physical State Lyophilized
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Rat IgG whole molecule was produced by repeated immunization with Rat IgG whole molecule in goat.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Anti-Rat IgG whole molecule antibody generated in goat detects specifically Rat IgG whole molecule. This secondary antibody anti-Rat is ideal for investigators who routinely perform immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels.

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$ 242.00
Cat# ASR3108
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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