F(ab')2 Anti-Sheep IgG (H&L) (Alkaline Phosphatase Conjugated) Pre-Adsorbed Secondary Antibody
Donkey Polyclonal, Alkaline Phosphatase (Calf Intestine)
|Description||F(ab')2 Anti-SHEEP IgG [H&L] (DONKEY) Antibody Alkaline Phosphatase Conjugated Min X Ch GP Ham Hs Hu Ms Rb & Rt Serum Proteins|
|Conjugate||Alkaline Phosphatase (Calf Intestine)|
|Application ||WB, E, IC|
|Application Note||ELISA 1:2,000-1:10,000|
Western Blot 1:500-1:2,500
|Physical State||Liquid (sterile filtered)|
|Host Isotype||IgG F(ab')2|
|Target Isotype||IgG (H&L)|
|Buffer||0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0|
|Immunogen||Sheep IgG whole molecule|
|Shipping Condition||Wet Ice|
|Purity||This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Donkey Serum, Sheep IgG and Sheep Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c), or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins.|
|Storage Condition||Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.|
|Precautions Note||This product is for research use only and is not intended for therapeutic or diagnostic applications.|
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Provided below are standard protocols that you may find useful for product applications.
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
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