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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   F(ab')2 Anti-Mouse IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody   

F(ab')2 Anti-Mouse IgG (H&L) (Phycoerythrin Conjugated) Pre-Adsorbed Secondary Antibody

Rabbit Polyclonal, R-Phycoerythrin (RPE)

Product Information
Description F(ab')2 Anti-MOUSE IgG (H&L) (RABBIT) Antibody Phycoerythrin conjugated Min X Human Serum Proteins
Host Rabbit
Conjugate R-Phycoerythrin (RPE)
Target Species Mouse
Reactivity Mouse
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note IF Microscopy 1:100-1:250
FlowCytometry 1:100-1:250
Physical State Lyophilized
Host Isotype IgG F(ab')2
Target Isotype IgG (H&L)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Immunogen Anti-Mosue IgG was produced by repeated immunization with Mouse IgG whole moleculein rabbit.
Reconstitution Volume 1.0 mL
Reconstitution Buffer Restore with deionized water (or equivalent)
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Ambient
Purity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.  No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Human Serum Proteins.
Storage Condition Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated (Pre-Adsorbed) was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated (Pre-Adsorbed) is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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$ 295.00
Cat# ASR3195
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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