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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Human TNF Alpha Secondary Antibody   

Anti-Human TNF Alpha Secondary Antibody

Rabbit Polyclonal, Unconjugated

  • IHC - Anti-Human TNF Alpha  Secondary Antibody ASR3287
    Immunohistochemistry using Rockland's polyclonal TNFa antibody showing staining of formalin/PFA-fixed paraffin-embedded sections of human artery tissue sections. Sections were fixed in formaldehyde and subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0). Slides were blocked for ten minutes with 1.5% serum.  Primary antibody was diluted 1:100 and incubated with samples for 24 hours at 4°C.  HRP-conjugated goat anti-rabbit antibody was used as the secondary antibody.
  • IF - Anti-Human TNF Alpha  Secondary Antibody ASR3287
    Fluorescent immunohistochemistry showing staining of human colon by Rockland's anti-TNF alpha (formalin/PFA-fixed paraffin-embedded sections). Samples were formaldehyde-fixed, then blocked in 10% serum for 2 hours at 20°C. The primary antibody was diluted 1:100 and incubated with the sample for 2 hours at 20°C. Alexa Fluor® 680 goat polyclonal secondary antibody was used diluted 1:5000.
  • WB - Anti-Human TNF Alpha  Secondary Antibody ASR3287
    Western blot using Rockland's Anti-Human TNF-a (RABBIT) Antibody. Membrane blocked in 1% BSA-TBS-T 30 min RT, Rb-a-TNF alpha added at 1:1000 in 1% BSA-TBS-T o/n 4°C, DyLight 649 Gt-a-Rb 611-143-122 added at 1:20,000 in MB-070 30 min RT.
Product Information
Description Anti-Human TNF alpha (RABBIT) Antibody
Host Rabbit
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:1,000-1:5,000
Western Blot 1:500-1:2,000
Physical State Liquid (sterile filtered)
Host Isotype Antiserum
Buffer None
Immunogen The whole rabbit serum was prepared by repeated immunizations with recombinant human TNFa produced in E.coli.
Stabilizer None
Additional Information
Shipping Condition Dry Ice
Purity This antiserum has been heated to 56°C for 30 minutes.  The antiserum is directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays.  In general, this antibody also detects primate TNFa in the same formats using similar dilutions.  The antibody does not recognize human TNGb (lymphotoxin).   This antiserum will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples.  This antibody is also useful for neutralization of human and primate TNFa activity in bioassays.  It does not neutralize the biological activity of lymphotoxin.  For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested.  A control of similarly diluted normal rabbit IgG is recommended.
Storage Condition Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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$ 1,019.00
Cat# ASR3287
Availability: 3-5 days
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