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>   home   >   Products   >   Secondary Antibodies   >   Unconjugated   >   Anti-Human IL-8 Secondary Antibody   

Anti-Human IL-8 Secondary Antibody

Rabbit Polyclonal, Unconjugated

  • WB - Anti-Human IL-8  Secondary Antibody ASR3289
    Western Blot of Rabbit anti-Human IL-8 Antibody. Lane 1: Recombinant Human IL-8. Load: 50 ng per lane. Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1,000 overnight at 4°C. Secondary antibody: HRP Goat-a-rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.
Product Information
Description Anti-Human IL-8 (RABBIT) Antibody
Host Rabbit
Conjugate Unconjugated
Target Species Human
Reactivity Human
Clonality Polyclonal
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Application Note ELISA 1:1,000-1:5,000
Western Blot 1:500-1:2,000
Immunochemistry 1:400-1:800ImmunoPrecipitation:1:400-1:800
Physical State Liquid (sterile filtered)
Host Isotype Antiserum
Buffer None
Immunogen The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.
Stabilizer None
Preservative None
Additional Information
Shipping Condition Dry Ice
Purity Anti-IL 8 antiserum has been heated to 56°C for 30 minutes.  The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA.  Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry.
Storage Condition Store anti-IL8 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Protein Information
Research Areas
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Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.

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$ 1,067.00
Cat# ASR3289
(40 western blots)
Availability: 3-5 days
Bulk Size
Seasonal Special on Bulk Order
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