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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ACTN4 Antibody (monoclonal) (M01)   

ACTN4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant ACTN4.

     
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    ACTN4 monoclonal antibody (M01), clone 4D10. Western Blot analysis of ACTN4 expression in PC-12 ( (Cat # AT1033a )
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    ACTN4 monoclonal antibody (M01), clone 4D10 Western Blot analysis of ACTN4 expression in Hela S3 NE ( (Cat # AT1033a )
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    ACTN4 monoclonal antibody (M01), clone 4D10. Western Blot analysis of ACTN4 expression in Raw 264.7 ( (Cat # AT1033a )
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    ACTN4 monoclonal antibody (M01), clone 4D10. Western Blot analysis of ACTN4 expression in MCF-7 ( (Cat # AT1033a )
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    ACTN4 monoclonal antibody (M01), clone 4D10. Western Blot analysis of ACTN4 expression in NIH/3T3 ( (Cat # AT1033a )
  • WB - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody (M01), clone 4D10.

    Lane 1: ACTN4 transfected lysate(104.9 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml]
  • IHC - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human lung adenocarcinoma. [antibody concentration 3 ug/ml]
  • IF - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20 ug/ml]
  • E - ACTN4 Antibody (monoclonal) (M01) AT1033a
    Detection limit for recombinant GST tagged ACTN4 is approximately 0.1ng/ml as a capture antibody.
  • SPECIFICATION
  • CITATIONS
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF, E
Primary Accession O43707
Other Accession NM_004924
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4D10
Calculated MW 104854 Da
Additional Information
Gene ID 81
Other Names Alpha-actinin-4, F-actin cross-linking protein, Non-muscle alpha-actinin 4, ACTN4
Target/Specificity ACTN4 (NP_004915, 592 a.a. ~ 701 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsACTN4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Background

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.

References

1.RNAi-mediated down-regulation of alpha-actinin-4 decreases invasion potential in oral squamous cell carcinoma.Yamada S, Yanamoto S, Yoshida H, Yoshitomi I, Kawasaki G, Mizuno A, Nemoto TK.Int J Oral Maxillofac Surg. 2009 Nov 11. [Epub ahead of print]

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$ 315.00
Cat# AT1033a
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