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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   AKAP8 Antibody (monoclonal) (M01)   

AKAP8 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant AKAP8.

  • WB - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.06 KDa) .
  • WB - AKAP8 Antibody (monoclonal) (M01) AT1089a
    AKAP8 monoclonal antibody (M01), clone 3D4 Western Blot analysis of AKAP8 expression in Hela S3 NE ( (Cat # AT1089a )
  • WB - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody (M01), clone 3D4.

    Lane 1: AKAP8 transfected lysate(76.2 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Immunoperoxidase of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3 ug/ml]
  • IF - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Immunoprecipitation of AKAP8 transfected lysate using anti-AKAP8 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with AKAP8 monoclonal antibody.
  • E - AKAP8 Antibody (monoclonal) (M01) AT1089a
    Detection limit for recombinant GST tagged AKAP8 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O43823
Other Accession NM_005858
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3D4
Calculated MW 76108 Da
Additional Information
Gene ID 10270
Other Names A-kinase anchor protein 8, AKAP-8, A-kinase anchor protein 95 kDa, AKAP 95, AKAP8, AKAP95
Target/Specificity AKAP8 (NP_005849, 551 a.a. ~ 662 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAKAP8 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is located in the nucleus during interphase and is distinctly redistributed during mitosis. This protein has a cell cycle-dependent interaction with the RII subunit of PKA.


Toward a confocal subcellular atlas of the human proteome. Barbe L, et al. Mol Cell Proteomics, 2008 Mar. PMID 18029348.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Beausoleil SA, et al. Nat Biotechnol, 2006 Oct. PMID 16964243.A-kinase-anchoring protein 95 functions as a potential carrier for the nuclear translocation of active caspase 3 through an enzyme-substrate-like association. Kamada S, et al. Mol Cell Biol, 2005 Nov. PMID 16227597.Large-scale characterization of HeLa cell nuclear phosphoproteins. Beausoleil SA, et al. Proc Natl Acad Sci U S A, 2004 Aug 17. PMID 15302935.

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$ 315.00
Cat# AT1089a
(40 western blots)
Availability: 7-10 days
Bulk Size
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