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ATPIF1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ATPIF1.

     
  • IF - ATPIF1 Antibody (monoclonal) (M01) AT1245a
    Immunofluorescence of monoclonal antibody to ATPIF1 on MCF-7 cell . [antibody concentration 10 ug/ml]
    detail
  • WB - ATPIF1 Antibody (monoclonal) (M01) AT1245a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.4 KDa) .
    detail
  • WB - ATPIF1 Antibody (monoclonal) (M01) AT1245a
    ATPIF1 monoclonal antibody (M01), clone 3E2 Western Blot analysis of ATPIF1 expression in MCF-7 ( (Cat # AT1245a )
    detail
  • WB - ATPIF1 Antibody (monoclonal) (M01) AT1245a
    Western Blot analysis of ATPIF1 expression in transfected 293T cell line by ATPIF1 monoclonal antibody (M01), clone 3E2.

    Lane 1: ATPIF1 transfected lysate(12 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - ATPIF1 Antibody (monoclonal) (M01) AT1245a
    Detection limit for recombinant GST tagged ATPIF1 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9UII2
Other Accession BC009677
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2b kappa
Clone Names 3E3
Calculated MW 12249 Da
Additional Information
Gene ID 93974
Other Names ATPase inhibitor, mitochondrial, Inhibitor of F(1)F(o)-ATPase, IF(1), IF1, ATPIF1, ATPI
Target/Specificity ATPIF1 (AAH09677, 1 a.a. ~ 106 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsATPIF1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.

References

Up-regulation of the ATPase inhibitory factor 1 (IF1) of the mitochondrial H+-ATP synthase in human tumors mediates the metabolic shift of cancer cells to a Warburg phenotype. S?nchez-Cenizo L, et al. J Biol Chem, 2010 Aug 13. PMID 20538613.Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.Mutation detection in candidate genes for benign familial infantile seizures on a novel locus. Li N, et al. Int J Neurosci, 2010 Mar. PMID 20374090.IF(1) distribution in HepG2 cells in relation to ecto-F(0)F (1)ATPsynthase and calmodulin. Contessi S, et al. J Bioenerg Biomembr, 2007 Aug. PMID 17851741.Identifying leukocyte gene expression patterns associated with plasma lipid levels in human subjects. Ma J, et al. Atherosclerosis, 2007 Mar. PMID 16806233.

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$ 350.00
Cat# AT1245a
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Availability: 7-10 days
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