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BLMH Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant BLMH.

  • WB - BLMH Antibody (monoclonal) (M02) AT1300a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - BLMH Antibody (monoclonal) (M02) AT1300a
    BLMH monoclonal antibody (M02), clone 4A2. Western Blot analysis of BLMH expression in K-562 ( (Cat # AT1300a )
  • E - BLMH Antibody (monoclonal) (M02) AT1300a
    Detection limit for recombinant GST tagged BLMH is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13867
Other Accession NM_000386
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4A2
Calculated MW 52562 Da
Additional Information
Gene ID 642
Other Names Bleomycin hydrolase, BH, BLM hydrolase, BMH, BLMH
Target/Specificity BLMH (NP_000377, 356 a.a. ~ 454 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsBLMH Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily.


Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.Variation in bleomycin hydrolase gene is associated with reduced survival after chemotherapy for testicular germ cell cancer. de Haas EC, et al. J Clin Oncol, 2008 Apr 10. PMID 18398146.Micronuclei frequency induced by bleomycin in human peripheral lymphocytes: correlating BLHX polymorphism with mutagen sensitivity. Maffei F, et al. Mutat Res, 2008 Mar 1. PMID 18082847.Meta-analysis of genetic variability in the beta-amyloid production, aggregation and degradation metabolic pathways and the risk of Alzheimer's disease. Llorca J, et al. Acta Neurol Scand, 2008 Jan. PMID 17854420.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.

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$ 315.00
Cat# AT1300a
(40 western blots)
Availability: 7-10 days
Bulk Size
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