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CHD4 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CHD4.

  • WB - CHD4 Antibody (monoclonal) (M01) AT1514a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
  • WB - CHD4 Antibody (monoclonal) (M01) AT1514a
    CHD4 monoclonal antibody (M01), clone 4H4 Western Blot analysis of CHD4 expression in Hela S3 NE ( (Cat # AT1514a )
  • WB - CHD4 Antibody (monoclonal) (M01) AT1514a
    Western Blot analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.

    Lane 1: CHD4 transfected lysate(220 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - CHD4 Antibody (monoclonal) (M01) AT1514a
    Immunoperoxidase of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. [antibody concentration 3 ug/ml]
  • E - CHD4 Antibody (monoclonal) (M01) AT1514a
    Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14839
Other Accession NM_001273
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4H4
Calculated MW 218005 Da
Additional Information
Gene ID 1108
Other Names Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4
Target/Specificity CHD4 (NP_001264, 1632 a.a. ~ 1730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCHD4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.


ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis. Li R, et al. EMBO J, 2009 Sep 16. PMID 19644445.Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications. Musselman CA, et al. Biochem J, 2009 Sep 25. PMID 19624289.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells. Helbling Chadwick L, et al. Chromosoma, 2009 Aug. PMID 19296121.Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF. Ishii H, et al. J Biol Chem, 2009 Mar 20. PMID 19158090.

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$ 315.00
Cat# AT1514a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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