CHD4 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a partial recombinant CHD4.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, E |
---|---|
Primary Accession | Q14839 |
Other Accession | NM_001273 |
Reactivity | Human |
Host | mouse |
Clonality | Monoclonal |
Isotype | IgG2a Kappa |
Clone Names | 4H4 |
Calculated MW | 218005 Da |
Gene ID | 1108 |
---|---|
Other Names | Chromodomain-helicase-DNA-binding protein 4, CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta, CHD4 |
Target/Specificity | CHD4 (NP_001264, 1632 a.a. ~ 1730 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Dilution | WB~~1:500~1000 |
Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
Precautions | CHD4 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein.
References
ZIP: a novel transcription repressor, represses EGFR oncogene and suppresses breast carcinogenesis. Li R, et al. EMBO J, 2009 Sep 16. PMID 19644445.Binding of the CHD4 PHD2 finger to histone H3 is modulated by covalent modifications. Musselman CA, et al. Biochem J, 2009 Sep 25. PMID 19624289.Defining the human deubiquitinating enzyme interaction landscape. Sowa ME, et al. Cell, 2009 Jul 23. PMID 19615732.The Mi-2/NuRD complex associates with pericentromeric heterochromatin during S phase in rapidly proliferating lymphoid cells. Helbling Chadwick L, et al. Chromosoma, 2009 Aug. PMID 19296121.Mi2beta shows chromatin enzyme specificity by erasing a DNase I-hypersensitive site established by ACF. Ishii H, et al. J Biol Chem, 2009 Mar 20. PMID 19158090.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.