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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CHTF18 Antibody (monoclonal) (M01)   

CHTF18 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant CHTF18.

  • WB - CHTF18 Antibody (monoclonal) (M01) AT1532a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.53 KDa) .
  • WB - CHTF18 Antibody (monoclonal) (M01) AT1532a
    CHTF18 monoclonal antibody (M01), clone 1F5 Western Blot analysis of CHTF18 expression in HeLa ( (Cat # AT1532a )
  • WB - CHTF18 Antibody (monoclonal) (M01) AT1532a
    Western Blot analysis of CHTF18 expression in transfected 293T cell line by CHTF18 monoclonal antibody (M01), clone 1F5.

    Lane 1: CHTF18 transfected lysate(107 KDa).
    Lane 2: Non-transfected lysate.
  • E - CHTF18 Antibody (monoclonal) (M01) AT1532a
    Detection limit for recombinant GST tagged CHTF18 is approximately 0.1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8WVB6
Other Accession BC018184
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 1F5
Calculated MW 107383 Da
Additional Information
Gene ID 63922
Other Names Chromosome transmission fidelity protein 18 homolog, hCTF18, CHL12, CHTF18, C16orf41, CTF18
Target/Specificity CHTF18 (AAH18184, 886 a.a. ~ 975 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsCHTF18 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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CHTF18, CHTF8 (MIM 613202), and DCC1 (DSCC1; MIM 613203) are components of an alternative replication factor C (RFC) (see MIM 600404) complex that loads PCNA (MIM 176740) onto DNA during S phase of the cell cycle (Merkle et al., 2003 [PubMed 12766176]; Bermudez et al., 2003 [PubMed 12930902]).


1.Two Different Replication Factor C Proteins, Ctf18 and RFC1, Separately Control PCNA-CRL4Cdt2-Mediated Cdt1 Proteolysis during S Phase and following UV Irradiation.Shiomi Y, Hayashi A, Ishii T, Shinmyozu K, Nakayama J, Sugasawa K, Nishitani H.Mol Cell Biol. 2012 Jun;32(12):2279-88. Epub 2012 Apr 9.2.Stable interaction between the human PCNA loader complex Ctf18-RFC and DNA polymerase {epsilon} is mediated by the cohesion specific subunits, Ctf18, Dcc1 and Ctf8.Murakami T, Takano R, Takeo S, Taniguchi R, Ogawa K, Ohashi E, Tsurimoto T.J Biol Chem. 2010 Sep 7. [Epub ahead of print]

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$ 315.00
Cat# AT1532a
(40 western blots)
Availability: 7-10 days
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