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DAF Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant DAF.

  • WB - DAF Antibody (monoclonal) (M01) AT1709a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - DAF Antibody (monoclonal) (M01) AT1709a
    DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa ( (Cat # AT1709a )
  • WB - DAF Antibody (monoclonal) (M01) AT1709a
    Western Blot analysis of CD55 expression in transfected 293T cell line by DAF monoclonal antibody (M01), clone 1G3.

    Lane 1: CD55 transfected lysate(41.4 KDa).
    Lane 2: Non-transfected lysate.
  • E - DAF Antibody (monoclonal) (M01) AT1709a
    Detection limit for recombinant GST tagged DAF is approximately 0.03ng/ml as a capture antibody.
  •  - DAF Antibody (monoclonal) (M01) AT1709a
    Proximity Ligation Analysis of protein-protein interactions between LCK and CD55. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-CD55 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P08174
Other Accession NM_000574
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1G3
Calculated MW 41400 Da
Additional Information
Gene ID 1604
Other Names Complement decay-accelerating factor, CD55, CD55, CR, DAF
Target/Specificity DAF (NP_000565, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsDAF Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
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This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.


1.A novel form of Total Internal Reflection Fluorescence Microscopy (LG-TIRFM) reveals different and independent lipid raft domains in living cells.Asanov A, Zepeda A, Vaca L.Biochim Biophys Acta. 2009 Oct 17. [Epub ahead of print]

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$ 315.00
Cat# AT1709a
(40 western blots)
Availability: 7-10 days
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