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>   home   >   Products   >   Primary Antibodies   >   E2F3 Antibody (monoclonal) (M01)   

E2F3 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant E2F3.

  • WB - E2F3 Antibody (monoclonal) (M01) AT1834a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.01 KDa) .
  • WB - E2F3 Antibody (monoclonal) (M01) AT1834a
    E2F3 monoclonal antibody (M01), clone 5F7 Western Blot analysis of E2F3 expression in COLO 320 HSR ( (Cat # AT1834a )
  • E - E2F3 Antibody (monoclonal) (M01) AT1834a
    Detection limit for recombinant GST tagged E2F3 is 0.1 ng/ml as a capture antibody.
  • E - E2F3 Antibody (monoclonal) (M01) AT1834a
    Detection limit for recombinant GST tagged E2F3 is 0.03 ng/ml as a capture antibody.
  •  - E2F3 Antibody (monoclonal) (M01) AT1834a
    Proximity Ligation Analysis of protein-protein interactions between MSH2 and E2F3 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-E2F3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession O00716
Other Accession NM_001949
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5F7
Calculated MW 49162 Da
Additional Information
Gene ID 1871
Other Names Transcription factor E2F3, E2F-3, E2F3, KIAA0075
Target/Specificity E2F3 (NP_001940, 336 a.a. ~ 425 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsE2F3 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.


Hypoxia inducible microRNA 210 attenuates keratinocyte proliferation and impairs closure in a murine model of ischemic wounds. Biswas S, et al. Proc Natl Acad Sci U S A, 2010 Apr 13. PMID 20308562.E2F3 is a mediator of DNA damage-induced apoptosis. Martinez LA, et al. Mol Cell Biol, 2010 Jan. PMID 19917728.Cell cycle genes and ovarian cancer susceptibility: a tagSNP analysis. Cunningham JM, et al. Br J Cancer, 2009 Oct 20. PMID 19738611.KIF14 and E2F3 mRNA expression in human retinoblastoma and its phenotype association. Madhavan J, et al. Mol Vis, 2009. PMID 19190782.MicroRNA-128 inhibits glioma cells proliferation by targeting transcription factor E2F3a. Zhang Y, et al. J Mol Med, 2009 Jan. PMID 18810376.

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$ 315.00
Cat# AT1834a
(40 western blots)
Availability: 7-10 days
Bulk Size
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