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E4F1 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant E4F1.

  • WB - E4F1 Antibody (monoclonal) (M03) AT1840a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) .
  • WB - E4F1 Antibody (monoclonal) (M03) AT1840a
    Western Blot analysis of E4F1 expression in transfected 293T cell line by E4F1 monoclonal antibody (M03), clone 4B10.

    Lane 1: E4F1 transfected lysate(83.5 KDa).
    Lane 2: Non-transfected lysate.
  • IF - E4F1 Antibody (monoclonal) (M03) AT1840a
    Immunofluorescence of monoclonal antibody to E4F1 on HeLa cell . [antibody concentration 10 ug/ml]
  • E - E4F1 Antibody (monoclonal) (M03) AT1840a
    Detection limit for recombinant GST tagged E4F1 is 0.1 ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q66K89
Other Accession NM_004424
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4B10
Calculated MW 83496 Da
Additional Information
Gene ID 1877
Other Names Transcription factor E4F1, 632-, E4F transcription factor 1, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, p120E4F, p50E4F, E4F1, E4F
Target/Specificity E4F1 (NP_004415.2, 685 a.a. ~ 784 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsE4F1 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.


The role of LANP and ataxin 1 in E4F-mediated transcriptional repression. Cvetanovic M, et al. EMBO Rep, 2007 Jul. PMID 17557114.E4F1 is an atypical ubiquitin ligase that modulates p53 effector functions independently of degradation. Le Cam L, et al. Cell, 2006 Nov 17. PMID 17110336.The LIM-only protein FHL2 is a negative regulator of E4F1. Paul C, et al. Oncogene, 2006 Sep 7. PMID 16652157.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.E4F1, a novel estrogen-responsive gene in possible atheroprotection, revealed by microarray analysis. Nakamura Y, et al. Am J Pathol, 2004 Dec. PMID 15579445.

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$ 315.00
Cat# AT1840a
(40 western blots)
Availability: 7-10 days
Bulk Size
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