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EEF1D Antibody (monoclonal) (M04)

Mouse monoclonal antibody raised against a partial recombinant EEF1D.

  • WB - EEF1D Antibody (monoclonal) (M04) AT1856a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.75 KDa) .
  • WB - EEF1D Antibody (monoclonal) (M04) AT1856a
    EEF1D monoclonal antibody (M04), clone 4B12. Western Blot analysis of EEF1D expression in human pancreas.
  • IF - EEF1D Antibody (monoclonal) (M04) AT1856a
    Immunofluorescence of monoclonal antibody to EEF1D on HeLa cell . [antibody concentration 10 ug/ml]
  • E - EEF1D Antibody (monoclonal) (M04) AT1856a
    Detection limit for recombinant GST tagged EEF1D is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P29692
Other Accession NM_032378
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4B12
Calculated MW 31122 Da
Additional Information
Gene ID 1936
Other Names Elongation factor 1-delta, EF-1-delta, Antigen NY-CO-4, EEF1D, EF1D
Target/Specificity EEF1D (NP_115754, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEEF1D Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit, delta, functions as guanine nucleotide exchange factor. It is reported that following HIV-1 infection, this subunit interacts with HIV-1 Tat. This interaction results in repression of translation of host cell proteins and enhanced translation of viral proteins. Several alternatively spliced transcript variants encoding multiple isoforms have been found for this gene. Related pseudogenes have been defined on chromosomes 1, 6, 7, 9, 11, 13, 17, 19.


Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride. Lei YX, et al. Biomed Environ Sci, 2010 Apr. PMID 20514991.BMP-6 promotes E-cadherin expression through repressing deltaEF1 in breast cancer cells. Yang S, et al. BMC Cancer, 2007 Nov 13. PMID 17997862.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.Medulloblastoma outcome is adversely associated with overexpression of EEF1D, RPL30, and RPS20 on the long arm of chromosome 8. De Bortoli M, et al. BMC Cancer, 2006 Sep 12. PMID 16968546.

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$ 315.00
Cat# AT1856a
(40 western blots)
Availability: 7-10 days
Bulk Size
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