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>   home   >   Products   >   Primary Antibodies   >   EIF5 Antibody (monoclonal) (M01)   

EIF5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant EIF5.

  • WB - EIF5 Antibody (monoclonal) (M01) AT1885a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (73.15 KDa) .
  • WB - EIF5 Antibody (monoclonal) (M01) AT1885a
    EIF5 monoclonal antibody (M01), clone 2E6-4C12 Western Blot analysis of EIF5 expression in Jurkat ( (Cat # AT1885a )
  • WB - EIF5 Antibody (monoclonal) (M01) AT1885a
    Western Blot analysis of EIF5 expression in transfected 293T cell line by EIF5 monoclonal antibody (M01), clone 2E6-4C12.

    Lane 1: EIF5 transfected lysate(49.2 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - EIF5 Antibody (monoclonal) (M01) AT1885a
    Immunoperoxidase of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1 ug/ml]
  • IF - EIF5 Antibody (monoclonal) (M01) AT1885a
    Immunofluorescence of monoclonal antibody to EIF5 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - EIF5 Antibody (monoclonal) (M01) AT1885a
    Detection limit for recombinant GST tagged EIF5 is approximately 1ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55010
Other Accession BC032866
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 2E6-4C12
Calculated MW 49223 Da
Additional Information
Gene ID 1983
Other Names Eukaryotic translation initiation factor 5, eIF-5, EIF5
Target/Specificity EIF5 (AAH32866, 1 a.a. ~ 431 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsEIF5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).


Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.The crystal structure of the carboxy-terminal domain of human translation initiation factor eIF5. Bieniossek C, et al. J Mol Biol, 2006 Jul 7. PMID 16781736.Structure of the eukaryotic initiation factor (eIF) 5 reveals a fold common to several translation factors. Conte MR, et al. Biochemistry, 2006 Apr 11. PMID 16584190.CK2 phosphorylation of eukaryotic translation initiation factor 5 potentiates cell cycle progression. Homma MK, et al. Proc Natl Acad Sci U S A, 2005 Oct 25. PMID 16227438.The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.

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$ 315.00
Cat# AT1885a
(40 western blots)
Availability: 7-10 days
Bulk Size
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