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ENO1 Antibody (monoclonal) (M01)Purified Mouse Monoclonal Antibody (Mab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AT1908a | 100 ug 400 ul | 7-10 days | $ 315.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ENO1 Antibody (monoclonal) (M01) - Product info | |
| Application | IF, WB
|
| Primary Accession | P06733 |
| Reactivity | Human |
| Concentration | 1mg/ml |
| Isotype | IgG1 Kappa |
| Clone Names | 8G8 |
| Calculated MW | 47169 Da |
ENO1 Antibody (monoclonal) (M01) - Additional info | |
| Gene ID 2023 | |
| Other Names ENO1L1, MBP-1, MPB1, NNE, PPHenolase 1, (alpha) | |
| Target/Specificity ENO1 (AAH22545, 1 a.a. ~ 435 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. | |
| Dilution WB~~1:500~1000 | |
| Format Clear, colorless solution in phosphate buffered saline, pH 7.2 . | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ENO1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ENO1 Antibody (monoclonal) (M01) - Related products
AP2879a: ENOA Antibody (N-term)
AP2879b: ENOA Antibody (C-term)
AP6526c: ENO1 Antibody (Center)
DC00833: Human ENO1 cDNA Clone
LY10917a: ENO1 Over-expression Lysate
BP2879a: ENOA Antibody (N-term) Blocking Peptide
BP2879b: ENOA Antibody (C-term) Blocking Peptide
BP6526a: ENO1 Antibody (N-term) Blocking Peptide
BP6526c: ENO1 Antibody (Center) Blocking Peptide
ENO1 Antibody (monoclonal) (M01) - Application data
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Immunofluorescence of monoclonal antibody to ENO1 (AT1908a) on HeLa cell . [antibody concentration 10 ug/ml]
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Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.
Lane 1: ENO1 transfected lysate(27 KDa).
Lane 2: Non-transfected lysate.
ENO1 Antibody (monoclonal) (M01) - Research Areas
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BACKGROUND
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
REFERENCES
SEDLIN forms homodimers: characterisation of SEDLIN mutations and their interactions with transcription factors MBP1, PITX1 and SF1. Jeyabalan J, et al. PLoS One, 2010 May 14. PMID 20498720.Identification of melanoma antigens using a Serological Proteome Approach (SERPA). Suzuki A, et al. Cancer Genomics Proteomics, 2010 Jan-Feb. PMID 20181627.MBP-1 suppresses growth and metastasis of gastric cancer cells through COX-2. Hsu KW, et al. Mol Biol Cell, 2009 Dec. PMID 19846662.Identification of ATP citrate lyase as a positive regulator of glycolytic function in glioblastomas. Beckner ME, et al. Int J Cancer, 2010 May 15. PMID 19795461.Cathepsin X cleaves the C-terminal dipeptide of alpha- and gamma-enolase and impairs survival and neuritogenesis of neuronal cells. Obermajer N, et al. Int J Biochem Cell Biol, 2009 Aug-Sep. PMID 19433310.
