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FZD5 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant FZD5.

  • WB - FZD5 Antibody (monoclonal) (M01) AT2131a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • WB - FZD5 Antibody (monoclonal) (M01) AT2131a
    Western Blot analysis of FZD5 expression in transfected 293T cell line by FZD5 monoclonal antibody (M01), clone 6A3.

    Lane 1: FZD5 transfected lysate(64.5 KDa).
    Lane 2: Non-transfected lysate.
  • IP - FZD5 Antibody (monoclonal) (M01) AT2131a
    Immunoprecipitation of FZD5 transfected lysate using anti-FZD5 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FZD5 MaxPab rabbit polyclonal antibody.
  • E - FZD5 Antibody (monoclonal) (M01) AT2131a
    Detection limit for recombinant GST tagged FZD5 is approximately 0.3ng/ml as a capture antibody.
  •  - FZD5 Antibody (monoclonal) (M01) AT2131a
    Proximity Ligation Analysis of protein-protein interactions between WNT5A and FZD5 HeLa cells were stained with anti-WNT5A rabbit purified polyclonal 1:1200 and anti-FZD5 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q13467
Other Accession NM_003468
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 6A3
Calculated MW 64507 Da
Additional Information
Gene ID 7855
Other Names Frizzled-5, Fz-5, hFz5, FzE5, FZD5, C2orf31
Target/Specificity FZD5 (ENSP00000354607, 72 a.a. ~ 161 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsFZD5 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.


Analysis of eight genes modulating interferon gamma and human genetic susceptibility to tuberculosis: a case-control association study. M?ller M, et al. BMC Infect Dis, 2010 Jun 7. PMID 20525402.A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of frizzled 5 (Fzd5). Terabayashi T, et al. J Biol Chem, 2009 Sep 25. PMID 19643732.Non-association between polymorphisms of the frizzled receptor genes and bone mineral density in postmenopausal Korean women. Kim JG, et al. J Korean Med Sci, 2009 Jun. PMID 19543507.High-density association study of 383 candidate genes for volumetric BMD at the femoral neck and lumbar spine among older men. Yerges LM, et al. J Bone Miner Res, 2009 Dec. PMID 19453261.The pseudoreceptor BMP and activin membrane-bound inhibitor positively modulates Wnt/beta-catenin signaling. Lin Z, et al. J Biol Chem, 2008 Nov 28. PMID 18838381.

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$ 315.00
Cat# AT2131a
(40 western blots)
Availability: 7-10 days
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