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>   home   >   Products   >   Primary Antibodies   >   GFI1 Antibody (monoclonal) (M01)   

GFI1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GFI1.

  • WB - GFI1 Antibody (monoclonal) (M01) AT2193a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.75 KDa) .
  • WB - GFI1 Antibody (monoclonal) (M01) AT2193a
    Western Blot analysis of GFI1 expression in transfected 293T cell line by GFI1 monoclonal antibody (M01), clone 3G8.

    Lane 1: GFI1 transfected lysate(45 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - GFI1 Antibody (monoclonal) (M01) AT2193a
    Immunoperoxidase of monoclonal antibody to GFI1 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml]
  • IF - GFI1 Antibody (monoclonal) (M01) AT2193a
    Immunofluorescence of monoclonal antibody to GFI1 on HeLa cell. [antibody concentration 10 ug/ml]
  • E - GFI1 Antibody (monoclonal) (M01) AT2193a
    Detection limit for recombinant GST tagged GFI1 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99684
Other Accession NM_005263
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3G8
Calculated MW 45297 Da
Additional Information
Gene ID 2672
Other Names Zinc finger protein Gfi-1, Growth factor independent protein 1, Zinc finger protein 163, GFI1, ZNF163
Target/Specificity GFI1 (NP_005254, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGFI1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Citations (0)

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This gene encodes a nuclear zinc finger protein that functions as a transcriptional repressor. This protein plays a role in diverse developmental contexts, including hematopoiesis and oncogenesis. It functions as part of a complex along with other cofactors to control histone modifications that lead to silencing of the target gene promoters. Mutations in this gene cause autosomal dominant severe congenital neutropenia, and also dominant nonimmune chronic idiopathic neutropenia of adults, which are heterogeneous hematopoietic disorders that cause predispositions to leukemias and infections. Multiple alternatively spliced variants, encoding the same protein, have been identified for this gene.


Tag-SNP analysis of the GFI1-EVI5-RPL5-FAM69 risk locus for multiple sclerosis. Alcina A, et al. Eur J Hum Genet, 2010 Jul. PMID 20087403.A variant allele of Growth Factor Independence 1 (GFI1) is associated with acute myeloid leukemia. Khandanpour C, et al. Blood, 2010 Mar 25. PMID 20075157.The growth factor independence-1 (Gfi1) is overexpressed in chronic myelogenous leukemia. Huang M, et al. Acta Haematol, 2010. PMID 19887785.Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase. Salipante SJ, et al. Mol Cell Biol, 2009 Aug. PMID 19506020.Gfi-1 represses CDKN2B encoding p15INK4B through interaction with Miz-1. Basu S, et al. Proc Natl Acad Sci U S A, 2009 Feb 3. PMID 19164764.

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$ 315.00
Cat# AT2193a
(40 western blots)
Availability: 7-10 days
Bulk Size
Seasonal Special on Bulk Order
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