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GIT2 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant GIT2.

  • WB - GIT2 Antibody (monoclonal) (M01) AT2201a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (77.33 KDa) .
  • WB - GIT2 Antibody (monoclonal) (M01) AT2201a
    GIT2 monoclonal antibody (M01), clone 3B5-B9 Western Blot analysis of GIT2 expression in Jurkat ( (Cat # AT2201a )
  • WB - GIT2 Antibody (monoclonal) (M01) AT2201a
    Western Blot analysis of GIT2 expression in transfected 293T cell line by GIT2 monoclonal antibody (M01), clone 3B5-B9.

    Lane 1: GIT2 transfected lysate(52.6 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - GIT2 Antibody (monoclonal) (M01) AT2201a
    Immunoperoxidase of monoclonal antibody to GIT2 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]
  • IF - GIT2 Antibody (monoclonal) (M01) AT2201a
    Immunofluorescence of monoclonal antibody to GIT2 on HeLa cell. [antibody concentration 20 ug/ml]
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q14161
Other Accession BC001379
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B5-B9
Calculated MW 84543 Da
Additional Information
Gene ID 9815
Other Names ARF GTPase-activating protein GIT2, ARF GAP GIT2, Cool-interacting tyrosine-phosphorylated protein 2, CAT-2, CAT2, G protein-coupled receptor kinase-interactor 2, GRK-interacting protein 2, GIT2, KIAA0148
Target/Specificity GIT2 (AAH01379, 1 a.a. ~ 471 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGIT2 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations ( 0 )


This gene encodes a member of the GIT protein family, which interact with G protein-coupled receptor kinases and possess ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. GIT proteins traffic between cytoplasmic complexes, focal adhesions, and the cell periphery, and interact with Pak interacting exchange factor beta (PIX) to form large oligomeric complexes that transiently recruit other proteins. GIT proteins regulate cytoskeletal dynamics and participate in receptor internalization and membrane trafficking. This gene has been shown to repress lamellipodial extension and focal adhesion turnover, and is thought to regulate cell motility. This gene undergoes extensive alternative splicing to generate multiple isoforms, but the full-length nature of some of these variants has not been determined. The various isoforms have functional differences, with respect to ARF GAP activity and to G protein-coupled receptor kinase 2 binding.


Distinct class of putative non-conserved promoters in humans: comparative studies of alternative promoters of human and mouse genes. Tsuritani K, et al. Genome Res, 2007 Jul. PMID 17567985.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931.Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Olsen JV, et al. Cell, 2006 Nov 3. PMID 17081983.GIT2 represses Crk- and Rac1-regulated cell spreading and Cdc42-mediated focal adhesion turnover. Frank SR, et al. EMBO J, 2006 May 3. PMID 16628223.The multifunctional GIT family of proteins. Hoefen RJ, et al. J Cell Sci, 2006 Apr 15. PMID 16598076.

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$ 315.00
Cat# AT2201a
(40 western blots)
Availability: 7-10 days
Bulk Size
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