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HCLS1 Antibody (monoclonal) (M02)

Mouse monoclonal antibody raised against a partial recombinant HCLS1.

  • WB - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) .
  • WB - HCLS1 Antibody (monoclonal) (M02) AT2329a
    HCLS1 monoclonal antibody (M02), clone 1A8 Western Blot analysis of HCLS1 expression in K-562 ( (Cat # AT2329a )
  • WB - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody (M02), clone 1A8.

    Lane 1: HCLS1 transfected lysate(54 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]
  • E - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Detection limit for recombinant GST tagged HCLS1 is approximately 0.1ng/ml as a capture antibody.
  • WB - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi ( (Cat # AT2329a )
  •  - HCLS1 Antibody (monoclonal) (M02) AT2329a
    Proximity Ligation Analysis of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P14317
Other Accession BC016758
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 1A8
Calculated MW 54014 Da
Additional Information
Gene ID 3059
Other Names Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1, LckBP1, p75, HCLS1, HS1
Target/Specificity HCLS1 (AAH16758, 266 a.a. ~ 355 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsHCLS1 Antibody (monoclonal) (M02) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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HS1 complexes with cytoskeleton adapters in normal and malignant chronic lymphocytic leukemia B cells. Muzio M, et al. Leukemia, 2007 Sep. PMID 17508001.A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, et al. Cell, 2006 May 19. PMID 16713569.Towards a proteome-scale map of the human protein-protein interaction network. Rual JF, et al. Nature, 2005 Oct 20. PMID 16189514.The coiled-coil domain is required for HS1 to bind to F-actin and activate Arp2/3 complex. Hao JJ, et al. J Biol Chem, 2005 Nov 11. PMID 16157603.Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry. Tao WA, et al. Nat Methods, 2005 Aug. PMID 16094384.

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$ 315.00
Cat# AT2329a
(40 western blots)
Availability: 7-10 days
Bulk Size
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